Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKNNLPIIALDFASAEETLAFLAPFQQEPLFVKVGMELFYQEGPSIVKQLKERNCELFLDLKLHDIPTTVNKAMKRLASLGVDLVNVHAAGGKKMMQAALEGLEEGTPAGKKRPSLIAVTQLTSTSEQIMKDELLIEKSLIDTVVHYSKQAEESGLDGVVCSVHEAKAIYQAVSPSFLTVTPGIRMSEDAANDQVRVATPAIAREKGSSAIVVGRSITKAEDPVKAYKAVRLEWEGIKS 3TR2 Chain:A ((5-233)) --DPKVIVAIDAGTVEQARAQINPLTPELCHLKIGSILFTRYGPAFVEELMQKGYRIFLDLKFYDIPQTVAGACRAVAELGVWMMNIHISGGRTMMETVVNALQSITL--KEKPLLIGVTILTSLDGSDLKT-LGIQEKVPDIVCRMATLAKSAGLDGVVCSAQEAALLRKQFDRNFLLVTPGIR----------RVMTPRAAIQAGSDYLVIGRPITQSTDPLKALEAIDKDI-----

General information: TITO was launched using: RESULT: Template: 3TR2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1110 -171387 -154.40 -782.59 target 2D structure prediction score : 0.78 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -154.40 2D Compatibility (Sec. Struct. Predict.) : 0.78 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.615

(partial model without unconserved sides chains): PDB file : Tito_3TR2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TR2-query.scw PDB file : Tito_Scwrl_3TR2.pdb: