Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMEKTFIMVKPDGVQRQLIGDILSRFERKGLQLAGAKLMRVTEQMAEKHYAEHQGKPFFGELVEFITSGPVFAMVWEGENVIEVTRQLIGKTNPKEALPGTIRGDYGMFVGKNIIHGSDSLESAEREINIFFKNEELVSYQQLMAGWIY 1BE4 Chain:C ((4-150)) --ERTFIAIKPDGVQRGLMGEIIKRFEQKGFRLVAMKFMRASEDLLKEHYIDLKDRPFFAGLVKYMHSGPVVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVGRNIIHGSDSVESAEKEIALWFRPEELVNYKSCAQNWIY

General information: TITO was launched using: RESULT: Template: 1BE4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 708 -73978 -104.49 -503.25 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain C : 0.88 3D Compatibility (PKB) : -104.49 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.88 QMean score : 0.511

(partial model without unconserved sides chains): PDB file : Tito_1BE4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1BE4-query.scw PDB file : Tito_Scwrl_1BE4.pdb: