Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MELYIITGASKGLGQAIALQALEKGHEVHALSRTKTDV----SH--K-KLTQHQIDLINLEEAEQQFETLLSSIDSDRYSGITLINNAGMVTPIKRAGEASLDELQRHYQLNLTAPVLLSQLFTKRFASYSGKKTVVNITSGAAKNPYKGWSAYCSSKAGLDMFTRTFGFEQEDEELPVNMISFSPGVMDTEMQAVIRSSSKKDFHHIERFRKLNETGSLRSPDFIAGTLLSLLEKG--TENGRIYDIKEFL 4HP8 Chain:A ((9-241)) GRKALVTGANTGLGQAIAVGLAAAGAEVVCAARRAPDETLDIIAKDGGNASALLIDFADPLAAKDSFTD---------AGFDILVNNAGIIRRA-DSVEFSELDWDEVMDVNLKALFFTTQAFAKELLAKGRSGKVVNIASLLSFQGGIRVPSYTAAKHGVAGLTKLLANEWA--AKGINVNAIAPGYIETNNTEALRAD----AARNKAILERIPAGRWGHSEDIAGAAVFLSSAAADYVHGAILNVD---

General information: TITO was launched using: RESULT: Template: 4HP8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1195 -18073 -15.12 -80.68 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -15.12 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.478

(partial model without unconserved sides chains): PDB file : Tito_4HP8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4HP8-query.scw PDB file : Tito_Scwrl_4HP8.pdb: