Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLNLQGKTALVTGSTSGIGKAIASSLAEEGAAVIINGRREEKVNQTIDELKTQHAEAVLYPAAFDLGTEEGCNELF----QAYPEVDILVNNLGIFEPAEYFDIPDDEWFRFFEVNIMSGVRLTRRYLHNMIEKKEGRVIFIASEAAIMPSQEMAHYSATKTMQLSISRSLAELATGTNVTVNTVMPGSTLTEGVETMLNSLYPGENLTVQEAEARFMKENRPTSIIQRLIRPEEIAHFVTFLSSPLSSAINGAALRADGGLVRSVF 2Q2Q Chain:F ((2-253)) ----LKGKTALVTGSTSGIGLGIAQVLARAGANIVLNGFGDPA--PALAEIARHGVKAVHHPA--DLSDVAQIEALFALAEREFGGVDILVNNAGIQHVAPVEQFPLESWDKIIALNLSAVFHGTRLALPGMRARNWGRIINIASVHGLVGSTGKAAYVAAKHGVVGLTKVVGLETATSNVTCNAICPGWVLTPL------------------AQHDLLAEKQPS---LAFVTPEHLGELVLFLCSEAGSQVRGAAWNVDGGWL----

General information: TITO was launched using: RESULT: Template: 2Q2Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 1316 -76344 -58.01 -330.49 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain F : 0.75 3D Compatibility (PKB) : -58.01 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.526

(partial model without unconserved sides chains): PDB file : Tito_2Q2Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Q2Q-query.scw PDB file : Tito_Scwrl_2Q2Q.pdb: