Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYIMKQSGWLELICGSMFSGKSEELIRRVKRATYAKQEVRVFKPVIDNRYSEAAVVSHNGTSMTSYAISSAADIWDHISESTDVVAVDEVQFFDQEIVEVLSSLADKGYRVIAAGLDMDFRGEPFGVVPNIMAIAESVTKLQAVCSVCGSPASRTQRLIDGKPASYDDPVILVGAAESYEARCRHHHEVPGKSKK 2WVJ Chain:D ((18-186)) ------RGQIQVILGPMFSGKSTELMRRVRRFQIAQYKCLVIKYAKDTRYS-------------ALPACLLRDVAQE-ALGVAVIGIDEGQFFP-DIVEFCEAMANAGKTVIVAALDGTFQRKPFGAILNLVPLAESVVKLTAVCMECFREAAYSKRL------GTEKEVEVIGGADKYHSVCR-----------

General information: TITO was launched using: RESULT: Template: 2WVJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 825 -105955 -128.43 -674.87 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain D : 0.77 3D Compatibility (PKB) : -128.43 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.450

(partial model without unconserved sides chains): PDB file : Tito_2WVJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2WVJ-query.scw PDB file : Tito_Scwrl_2WVJ.pdb: