Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKILIAEHYEELCKLSAAIIKEQIQAKKDAVLGLATGSTPVGLYKQLISDYQAGEIDFSKVTTFNLDEYAGLSPSHPQSYNHFMHEHLFQHINMQPDHIHIPQGDNPQLEAACKVYEDLIRQAGGIDVQILGIGANGHIGFNEPGSDFEDRTRVVKLSESTIQANARFFGGDPVLVPRLAISMGIKTIMEFSKHIVLLASGEEKADAIQKMAEGPVTTDVPASILQKHNHVTVIADYKAAQKLKSASFS 2RI0 Chain:A ((2-234)) MKTIKVKNKTEGSKVAFRMLEEEIT-FGAKTLGLATGSTPLELYKEIRE----SHLDFSDMVSINLDEYVGLSADDKQSYAYFMKQNLFAAKPFKK--SYLPNGLAADLAKETEYYDQILAQ-YPIDLQILGIGRNAHIGFNEPGTAFSSQTHLVDLTPSTIAANSRFFEK-AEDVPKQAISMGLASIMSAK-MILLMAFGEEKAEAVAAMVKGPVTEEIPASILQTHPKVILIVDEKAGAGI------

General information: TITO was launched using: RESULT: Template: 2RI0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1287 -153750 -119.46 -659.87 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.82 3D Compatibility (PKB) : -119.46 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.634

(partial model without unconserved sides chains): PDB file : Tito_2RI0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2RI0-query.scw PDB file : Tito_Scwrl_2RI0.pdb: