Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSEQKPVQWASKIGFVMAAAGSAIGLGAIWKFPYVAGTNGGGAFFLIFVLFTILLGYPLLVGEFIFGRRNQTNAIDA----YKKE--APRSAWFLTGWIGVAACFLVLSFYSVIGGWILLYIVKTASGSLSGLSQ-------AQYGALFASIIQ------N--PVQTLA--AQLVFMALTVLVVARGVQKGIERVSAVMMPILFLLFILLVLRSLTLN----GAMEGVKFLLVPHFGDL-TPESILFALGQAFFTLTLGVSVMVTYSSYLPKTQNIPRSAASIVLMNIIVTLLAGL--AIFPAVFSFG------FQPNEGPTLLFTVLPAVFEQLPFGTLFFIGFLVAFLFAALTSAFSMVEIIVATIGKGDEKKRKK-LSWTSGLLIFLVGIPCCLSYGVLSDVHLFGKTFFDIADFTVSNVLMPSGALLISLFIPLKISKSELLAEMRNGSNAGKAFFYTWFYLLRFIVPLAIIIVFLNLIGILSF 2A65 Chain:A ((5-468)) -----REHWATRLGLILAMAGNAVGLGNFLRFPVQAAENGGGAFMIPYIIAFLLVGIPLMWIEWAMGRYGGAQGHGTTPAIFYLLWRN--RFAKILGVFGLWIPLVVAIYYVYIESWTLGFAIKFLVGLVPEPPPTDPDSILRPFKEFLYSYIGVPKGDEPILKPSLFAYIVFLITMFINVSILIRGISKGIERFAKIAMPTLFILAVFLVIRVFLLETPNGTAADGLNFLWTPDFEKLKDPGVWIAAVGQIFFTLSLGFGAIITYASYVRKDQDIVLSGLTAATLNEKAEVILGGSISIPAAVAFFGVANAVAIAKAGAFNLGFITLPAIFSQTAGGTFLGFLWFFLLFFAGLTSSIAIMQPMIAFLEDELKLSRKHAVLWTAAIVFFSAHLVMFLNK------------SLDEMDFWAGTIGVVFFGLTELIIFFWIFGADKAWEEINRGGI--IKVPRIYYYVMRYITPAFLAVLLVVWA-----

General information: TITO was launched using: RESULT: Template: 2A65.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2385 -423200 -177.44 -995.76 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -177.44 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.425

(partial model without unconserved sides chains): PDB file : Tito_2A65.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2A65-query.scw PDB file : Tito_Scwrl_2A65.pdb: