Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAVKKVVTHPAEVLETPAETVTVFDKKLKKLLDDMYDTMLEMDGVGLAAPQIGILKRAAVVEI-GDDRGRIDLVNPEILEKSGEQTGIE-GCLSFPNVYGDVTRADYVKVRAFNRQGKPFILEARGFLARAVQHEMDHLDGVLFTSKISKYYTEDELADMEG 4WXK Chain:D ((2-148)) -TALNVLIYPDDHLKVVCEPVTEVNDAIRKIVDDMFDTMYQEKGIGLAAPQVDILQRIITIDVEGDKQNQFVLINPEILASEGE-TGIEEGCLSIPGFRALVPRKEKVTVRALDRDGKEFTLDADGLLAICIQHEIDHLNGILFVDYLS-------------

General information: TITO was launched using: RESULT: Template: 4WXK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 724 -78203 -108.02 -539.33 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain D : 0.83 3D Compatibility (PKB) : -108.02 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.515

(partial model without unconserved sides chains): PDB file : Tito_4WXK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4WXK-query.scw PDB file : Tito_Scwrl_4WXK.pdb: