Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKIYDAANWSKHE-D-DFTQMFYNQNVKQFWLPEEIALNGDLLTWKYLGKNEQDTYMKVLAGLTLLDTEQGNTGMPIVAEHVDGHQRKAVLNFMAMMENAVHAKSYSNIFMTLAPTETINEVFEWVKQNKYLQKKAQMIVGLYKAIQKDDEISLFKAMVASVYLESFLFYSGFYYPLYFYGQGKLMQSGEIINLILRDEAIHGVYVGLLAQEIYNKQTEEKKAELREFAIDLLNQLYENELEYTEDLYDQVGLSHDVKKFIRYNANKALMNLGFDPYFEEE--DINPIVLNGLNTKTKSHDFFSMKGNGYKKATVEPLKDDDFYFEDEKEQI 4N83 Chain:A ((3-287)) --TYYKAINW--NAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDWRKLSHKEKDLVGKVFGGLTLLDTLQSESGVDALRKDVRTAHEEAVFNNIQFME-SVHAKSYSSIFSTLNTKSEIDEIFAWTNTNPYLQKKAEIINEIYLNG------TALEKKIASVFLETFLFYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLAFNELPEDEQEKLKEWMYDLLYTLYENEEGYTESLYDTVGWTEEVKTFLRYNANKALMNLGQDPLFPDSADDVNPIVMNGIST-------------------------------------

General information: TITO was launched using: RESULT: Template: 4N83.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1252 -130323 -104.09 -463.78 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.82 3D Compatibility (PKB) : -104.09 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.562

(partial model without unconserved sides chains): PDB file : Tito_4N83.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4N83-query.scw PDB file : Tito_Scwrl_4N83.pdb: