Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGDSILFTVKNEHMALITLNRPQAANALSAEMLRNLQMIIQEIEFNSNIRCVILTGTGEKAFCAGADLKERIKLKEDQVLESVSLIQRTAALLDALPQPVIAAINGSALGGGLELALACDLRIATEAAVLGLPETGLAIIPGAGGTQRLPRLIGRGKAKEFIYTGRRVTAHEAKEIGLVEHVTAPCDLMPKAEELAAAISANGPIAVRQAKFAINKGLETDLATGLAIEQKAYEQTIPTKDRREGLQAFQEKRRAVYKGI 3PZK Chain:C ((10-250)) ---------RDQRVGIITLNRPQALNALNSQVMNEVTSAATELDDDPDIGAIIITGSA-KAFAAGADIKEMADLTFADAFTADFFA--TWGKLAAVRTPTIAAVAGYALGGGCELAMMCDVLIAADTAKFGQPEIKLGVLPGMGGSQRLTRAIGKAKAMDLILTGRTMDAAEAERSGLVSRVVPADDLLTEARATATTISQMSASAARMAKEAVNRAFESSLSEGLLYERRLFHSAFATEDQSEGMAAFIEKR-------

General information: TITO was launched using: RESULT: Template: 3PZK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1174 -128173 -109.18 -531.84 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain C : 0.82 3D Compatibility (PKB) : -109.18 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.462

(partial model without unconserved sides chains): PDB file : Tito_3PZK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3PZK-query.scw PDB file : Tito_Scwrl_3PZK.pdb: