Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNQKHSSDFVVIKAVEDGVNVIGLTRGTDTKFHHSEKLDKGEVIIAQFTEHTSAIKVRGEALIQTAYGEMKSEKK 3ZTE Chain:K ((10-77)) -------DFVVIKAVEDGVNVIGLTRGTDTRFHHSEKLDKGEVMICQFTEHTSAIKVRGEALIQTANGEMKSESK

General information: TITO was launched using: RESULT: Template: 3ZTE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain K - contact count / total energy / energy per contact / energy per residue : 277 -15553 -56.15 -228.71 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain K : 0.95 3D Compatibility (PKB) : -56.15 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.95 QMean score : 0.691

(partial model without unconserved sides chains): PDB file : Tito_3ZTE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ZTE-query.scw PDB file : Tito_Scwrl_3ZTE.pdb: