Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRHALITAGSKGLGRKVTETLLAKGYSVTVNYRQDEEAVSRLKEACPDCLD-RLQFVKGDVTKKEDLLRIAEAALNRFGRIDFLINNAGPYIFERKKLADYTDDEWYGMLEGNLSAVFHLFKAVIPIMRKQQFGRIITYGFQGAAHAPGWL---HRSAFGAAKVGLASLTKTIAIEEAEFGITANMVCPGDIVGDMKEASI-----------EEARMRIGKEKTPIGRSGTGEDIARIIAFLCEENSDLVTGTVIEATGGLNVINKNQTT 2ZTM Chain:A ((7-256)) ---AVVTGSTSGIGLGIATALAAQGADIVLNGFGDAAEIEKVRAGLAAQHGVKVLYDGADLSKGEAVRGLVDNAVRQMGRIDILVNNAG--IQHTALIEDFPTEKWDAILALNLSAVFHGTAAALPHMKKQGFGRIINI-----ASAHGLVASANKSAYVAAKHGVVGFTKVTALETAGQGITANAICPGWVRSPLVEKQISALAEKNGVDQETAARELLSEKQPSLQFVTPEQLGGTAVFLASDAAAQITGTTVSVDGG----------

General information: TITO was launched using: RESULT: Template: 2ZTM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1217 -50683 -41.65 -215.67 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -41.65 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.485

(partial model without unconserved sides chains): PDB file : Tito_2ZTM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZTM-query.scw PDB file : Tito_Scwrl_2ZTM.pdb: