Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MELYIITGASKGLGQAIALQALEKGHEVHALSRTKTDV--SH---KK-LTQHQIDLINLEEAEQQFETLLSSIDSDRYSGITLINNAGMVTPIKRAGEASLDELQRHYQLNLTAPVLLSQLFTKRFASYS---GKKTVVNITSGAAKNPYKGWSAYCSSKAGLDMFTRTFGFEQEDEELPVNMISFSPGVMDTEMQAVIRSSSKKDFHHIERFRKLNETGSLRSPDFIAGTLLSLLEKG--TENGRIYDIKEFL 3N74 Chain:A ((5-247)) GKVALITGAGSGFGEGMAKRFAKGGAKVVIVDRDKAGAERVAGEIGDAALAVAADISKEADVDAAVEAALSK----FGKVDILVNNAGIGHKPQNAELVEPEEFDRIVGVNVRGVYLMTSKLIPHFKENGAKGQECVILNVASTGAGRPRPNLAWYNATKGWVVSVTKALAIELA--PAKIRVVALNPV---------------------------IPMGRLLKPDDLAEAAAFLCSPQASMITGVALDVD---

General information: TITO was launched using: RESULT: Template: 3N74.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1111 38670 34.81 186.81 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : 34.81 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.481

(partial model without unconserved sides chains): PDB file : Tito_3N74.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3N74-query.scw PDB file : Tito_Scwrl_3N74.pdb: