Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRIKEHLKQLKPYQPGKPIEAVKSEYGLDKVVKLASNENPYGCSEAAKEALH-HEIQQLALYPDGYSAALRTRLSKHLNVSETSLIFGNGSDEIIQIICRAFLNNKTNTITAAPTFPQYKHNAVIEGAEVREIALRPDGSHDLDAMLEAID-EQTQVVWICSPNNPTGTYTSEGELLAFLERVPSRVLVVLDEAYYEYVTAEDYPETVPLLSKYSNLMILRTFSKAYGLAALRVGYGIADENLIRQIEPAREPFNTSRLGQAAAIAALDDQAFIASCVEQNNAGLQQYYDFAKTHGLKCYPSQTNFVLIDFK-RP-AD-ELFQALL-EKGYIVR-SGNALGFPTSLRITIGTKEQNEEILAILAEIL 4WBT Chain:A ((9-370)) -RFTPLIQSL-----FVGPEALERQHGRKIAARIGANESGFGPAPSVLLAIRQAA-GDTWKYADPENHDLKQALARHLGTSPANIAIGEGIDGLLGQIVRLVVEAGAPVVTSLGGYPTFNYHVAGHGGRLVTVPYAD-DREDLEGLLAAVGRENAPLVYLANPDNPMGSWWPAERVVAFAQALPETTLLVLDEAY-ETAPRDALPPIESL-IDKPNVIRARTFSKAYGLAGARIGYTLSTPGTAQAFDKIRNHFGMSRIGVAAAIAALADQDYLKEVTLKIANSRQRIGRIAADSGLAPLPSATNFVAVDCGKDASYARAIVDRLMSDHGIFIRMPGVAP-LNRCIRISTAPDAEMDLLAAALPEVI

General information: TITO was launched using: RESULT: Template: 4WBT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1930 10949 5.67 31.37 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : 5.67 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.534

(partial model without unconserved sides chains): PDB file : Tito_4WBT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4WBT-query.scw PDB file : Tito_Scwrl_4WBT.pdb: