Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPINSFENYPMSWKPSIDKAEKPIYKALAGQLEQDILNGVLLPGTKLPPQRELADYLDLNVSTISKAFKVCELKGLLSATVGSGTFVSYDALSNAYLL-----E----DTKPTHLIEMGAILPDHASYEP--LLYQLKNMVHEEDYEKWFSYGRAGESLWQKDAAVKLIQR-GGFETSVDHILFANGGQNAIAATLASLCKPGDRIGVDHHTYPGLKTVASLLSVQIVPIKSENDEMSPESLEYACKNENIKGLYLIPDYHNPTTSFMSVENRRMVADIVKKY-NLFVIEDASYHLLNKNP--LPALASFA-PQQVIHIASLSKSLAPGLRLAYVAVPRQYKEPISKALYNLNITVSPLLAELTARTIVSNQFEVLIESHREQTIRRNQLVNRYVAGY-----TCLGVETGIFRWLLLPGKMSGAEFEELAARLGVQVYAAERFVVGNSCPERAVRVSVCAPKTLEELEQGLMILRRLLDDLT 4TV7 Chain:A ((14-463)) ---------------------DYIYQQIYQKLKKEILSRNLLPHSKVPSKRELAENLKVSVNSVNSAYQQLLAEGYLYAIERKGFFVEELDMFSA---ALPDDLKEIHI-----WISFSHM--DTDHFPIKSWFRCEQKAASRS-Y-----MSHPQGIYEVRAAITRLISLTRGVKCRPEQMIIGAGTQVLMQLLTE-LLPKEAVYAMEEPGYRRMYQLLKNAGKQVKTIMLDEKGMSIAEITR----QQPDVLVTTPSHQFPSGTIMPVSRRIQLLNWAAEEPRRYIIEDDYDSEFTYDVDSIPALQSLDRFQNVIYMGTFSKSLLPGLRISYMVLPPELLRAYKQR-GYDLQTCSSLTQLTLQEFIESGEYQKHIKKMKQHYKEKRERLITALEAEFSGEVTVKGANAGLHFVTE-DTRRTEQDILSHAAGLQLEIFGMSRF-------RPALIIGFAR-LKEEDIQEGVQR---------

General information: TITO was launched using: RESULT: Template: 4TV7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1945 -213445 -109.74 -532.28 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -109.74 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.533

(partial model without unconserved sides chains): PDB file : Tito_4TV7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4TV7-query.scw PDB file : Tito_Scwrl_4TV7.pdb: