Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNAKTKLVTDRIRLRCMEDRDQATLFGLLFNDPDVMTYYSGLKDKRQTREWVNWNQRNEKGYGVSLWIAEDKRTGEFLGQCGIVPQQIENQTVMEIGYMFARRHWGNGYAQEAARACLDYGFNERQFGKMAALIDPGNKASIRVAEKIGMHYSKTIRKWNK---------PIAVYERKSYN- 4MI4 Chain:A ((26-195)) --------NSQLTLRALE-RGDLRFIHNLNNNRNIMSYW-FEEPYESFDELEELYNKHIHDNAERRFVVEDA-QKNLIGLVELIEINYIHR-SAEFQIIIAPEHQGKGFARTLINRALDYSFTILNLHKIYLHVAVENPKAVHLYEECGFVEEGHLVEEFFINGRYQDVKRMYILQSKYLNR

General information: TITO was launched using: RESULT: Template: 4MI4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 625 -91799 -146.88 -573.74 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -146.88 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.390

(partial model without unconserved sides chains): PDB file : Tito_4MI4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MI4-query.scw PDB file : Tito_Scwrl_4MI4.pdb: