Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFMFEKPHGMRDTLPGLYETKKKVRRSLTDLIDKWGYQFMETPTLEFYDTVGVQSAIEEQQLFKLLDQDGKTLVLRPDMTGPIARVAASKLLKHGHPLRVGYAANVFRAQEREGGRPAEFEQVGVELIGDGT-TSADAEVIALVVGALKNAGLASFKIAIGHAGIADALFVEVLGNVERADVLRRFLYEKNYVGYREH-VKSL---PLSSIDKSRLLELLELRGGIEVCGRAE---EIVDSAQGKSVVDELKALWDILEDYGCTENVRLDLNMVSHMSYYTGILFEVYAENV---GFVIGSGGRYNKLLGHFDS---PAPATGFGLRIDRLIEALHMKDE-----P-C-EIDAV-IFSKEQRAQ-AIAYANEERMKGNKVVLQDLS--GIE-NIDQMTKSFANVTYFIGARKEEQNG 4G84 Chain:A ((13-430)) --VLKTPKGTRDYSPRQMAVREKVFDVIIRCFKRHGAEVIDTPVFELKETLMGKYG----LIYDLKDQGGELLSLRYDLTVPFARYLAMNK---LTNIKRYHIAKVYRRDN----RYREFYQCDFDIAGNFDPMIPDAECLKIMCEILSSLQIGDFLVKVNDRRILDGMFAICGVSDSKFRTICSSVDKLDKVSWEEVKNEMVGEKGLAPEVADRIGDYVQQHGGVSLVEQLLQDPKLSQNKQALEGLGDLKLLFEYLTLFGIDDKISFDLSLARGLDYYTGVIYEAVLLQTPLGVGSVAAGGRYDGLVGMFDPKGRKVPCVGLSIGVERIFSIVEQRLEALEEKIRTTETQVLVASAQKKLLEERLKLVSELWDAGIKAELLYKKNPKLLNQLQYCEEAGIPLVAIIGEQELK---

General information: TITO was launched using: RESULT: Template: 4G84.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1825 -27534 -15.09 -73.42 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -15.09 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.456

(partial model without unconserved sides chains): PDB file : Tito_4G84.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4G84-query.scw PDB file : Tito_Scwrl_4G84.pdb: