Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEEKEILWNEAKAFIAACYQELGK-EEEVKDRLADIKSEIDLTGSYVHTKEELEHGAKMAWRNSNRCIGRLFWNSLNVIDRRDVRTKEEVRDALFHHIETATNNGKIRPTITIFPPEEKGEKQVEIWNHQLIRYAGYESDGERIGDPASCSLTAACEELGWRGERTDFDLLPLIFRMKGDEQPVWYELPRSLVIEVPITHPDIEAFSDLELKWYGVPIISDMKLEVGGIHYNAAPFNGWYMGTEIGARNLADEKRYDKLKKVASVIGIAADYNTDLWKDQALVELNKAVLHSYKKQGVSIVDHHTAASQFKRFEEQEEEAGRKLTGDWTWLIPPISPAATHIFHRSYDNSIVKPNYFYQDKPYE 2FLQ Chain:A ((11-368)) -QHDEQLMTKAEQFIIASYRELGKSEQEIKRRVNEIRWEVEQTGTYRHTYEELSYGAKMAWRHSNRCIGRLFWQSLHVIDAREAVTEEEVFSYLFHHIEVATNGGKIRPTITIFRPN----GEVRIWNHQLIRYAGYETEEGIIGDSSSLTFTRACEQLGWKGEKTPFDVLPLVIQV-GGQKPVWTPIPKELVLEVPIEHPEFPWFRDLQLKWYAVPIISDMCLEIGGIRYMAAPFNGWYMGTEIGARNFADDYRYNMLPKVASCMGLDTNSNASLWKDKALVELNIAVLYSYKKAGVSIVDHHTAARQFQLFEQQEKAAGRHVTGDWTWLIPPLSPATTHIFHRSYDNTMMLPNFFYQDRPYE

General information: TITO was launched using: RESULT: Template: 2FLQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1844 -202353 -109.74 -566.82 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.90 3D Compatibility (PKB) : -109.74 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.90 QMean score : 0.521

(partial model without unconserved sides chains): PDB file : Tito_2FLQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FLQ-query.scw PDB file : Tito_Scwrl_2FLQ.pdb: