Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----MLEVKLPGLDLKNPIIPASGCF-GFGKEFSRFYDLSCLGAIMIKATT--KEPRFGNPTPRVAE--TGAGMLNAIGLQNPGLDSVLHHELPWLEQ----FDTPIIANVAGSQ-VDDYVEVAEHISKAPNVHALELNISCPNVKT-GGIAFGTNPEMAADLTKAVKEVSDVPVYVKLSPNV-ANITEIALAIEEAGADGLTMINTLIG-MRLDLKTGKPIL--ANKTGGLSGPAVKPVAIRMVYEVSQMVN-IPIIGMGGVQTAEDALEFLLAGASAVAVGTANFVN-PFACPEIIEQLPSVLLQYGYQSIEECIGRSWNHEKQPAHHRA 1EP3 Chain:A ((1-311)) MTENNRLSVKLPGLDLKNPIIPASG-CFGFGEEYAKYYDLNKLGSIMVKATTLH--PRFGNPTPRVAETA--SGMLNAIGLQNPGLEVIMTEKLPWLNENFPE--LPIIANVAGS-EEADYVAVCAKIGDAANVKAIELNISCPNVKHG-GQAFGTDPEVAAALVKACKAVSKVPLYVKLSPNVT-DIVPIAKAVEAAGADGLTMINTLM-GVRFDLKTRQPILAN--ITGGLSGPAIKPVALKLIHQVAQDV-DIPIIGMGGVANAQDVLEMYMAGASAVAVGTANFA-DPFVCPKIIDKLPELMDQYRIESLESLIQEVKEGKK-------

General information: TITO was launched using: RESULT: Template: 1EP3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1739 -223045 -128.26 -771.78 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.88 3D Compatibility (PKB) : -128.26 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.88 QMean score : 0.511

(partial model without unconserved sides chains): PDB file : Tito_1EP3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1EP3-query.scw PDB file : Tito_Scwrl_1EP3.pdb: