Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTQFDKQYNSIIKDIINNGISDEEFDVRTKWDSDGTPAHTLSVISKQMRFD-NSEVPILTTKKVAWKTAIKELLWIWQLKSNDVNDLNMMGVHIWDQ------------WKQEDGTIGHAYGFQ---LGKKNRSL----NGEKVDQVDYLLHQLKNNPSSRRHITMLWNPDELDAMALTPCVYETQWYVKHGKLHLEVRARSNDMALGNPFNVFQYNVLQRMIAQVTGYELGEYIFNIGDCHVYTRHIDNLKIQMEREQFEAPELWINPEVKDFYDFTIDDFKLINYKHGDKLLFEVAV 3H9K Chain:C ((32-306)) ------QYLGQIQHILRCGVRKD----------DRTGTGTLSVFGMQARYSLRDEFPLLTTKRVFWKGVLEELLWFIK-GSTNAKELSSKGVKIWDANGSRDFLDSLGFSTREEGDLGPVYGFQWRHFGAEYRDMESDYSGQGVDQLQKVIDTIKTNPDDRRIIMCAWNPRDLPLMALPPCHALCQFYVVNSELSCQLYQRSGDMGLGVPFNIASYALLTYMIAHITGLKPGDFIHTLGDAHIYLNHIEPLKIQLQREPRPFPKLRILRKVEKIDDFKAEDFQIEGYNPHPT-------

General information: TITO was launched using: RESULT: Template: 3H9K.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1230 4790 3.89 18.78 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain C : 0.77 3D Compatibility (PKB) : 3.89 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.598

(partial model without unconserved sides chains): PDB file : Tito_3H9K.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3H9K-query.scw PDB file : Tito_Scwrl_3H9K.pdb: