Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKWIVLFLVLIAAAISIFVYVSTGSEKPFYNDINLTQYQKEVDSKKPKFIYVYETSCPPCQEIKPELNEVIKKEK--LKVQALNIEEKENYNTEFLDKYNLNKTPTILYYKDGKEKDRLEGYRSASQIEKFFDKNGDR 3TCO Chain:A ((5-108)) -----------------------------VTLVLTEENFDEVIRNNKLVLVDCWAEWCAPCHLYEPIYKKVAEKYKGKAVFGRLNVDEN----QKIADKYSVLNIPTTLIFVNGQLVDSLVGAVDEDTLESTVNKYL--

General information: TITO was launched using: RESULT: Template: 3TCO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 443 -6762 -15.26 -66.29 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -15.26 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.577

(partial model without unconserved sides chains): PDB file : Tito_3TCO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TCO-query.scw PDB file : Tito_Scwrl_3TCO.pdb: