Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKYTMNEMVDITKDMLNKRGVMIEDIARIVQKLQEKYNPNLPLSVCMENVEKVLNKREIIHAVLTGLALDQLAEQKLLPEPLQHLVETDEPLYGIDEIIPLSIVNVYGSIGLTNFGYLDKEKIGIIKELDESPD-GIHTFLDDIVAALAAAAASRIAHTHQDLQDEEKEQDEKPVVS 1RFZ Chain:A ((4-164)) MSEFIMNNLEQTARRWLEERGVTVEKIAELVYYLQSKYHPDLTMEECIENVNRVISKREVQNAILTGIQLDKLAEDGRLDEPLQSIIRRDEGLYGVDEILALSIVNVYGSIGFTNYGYIDKQKPGILQYLNDKSTGKCNTFLDDIVGAIAAAASSRLAHRA-----------------

General information: TITO was launched using: RESULT: Template: 1RFZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 759 -97235 -128.11 -607.72 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.88 3D Compatibility (PKB) : -128.11 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.88 QMean score : 0.500

(partial model without unconserved sides chains): PDB file : Tito_1RFZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1RFZ-query.scw PDB file : Tito_Scwrl_1RFZ.pdb: