Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQLQVMNSPFNQEQAELLNRLLPTLTESQKIWLSGYLSAQSVSAQEAAGTPAAAVSAEAPAPAVSKEVTVLYGSQTGNAQGLAENAGKQLEQSGFQVTVSSMSDFKPNQLKKVTNLLIVVSTHGEGEPPDNALSFHEFLHGRRAPKLEDLRFSVLALGDSSYEFFCQTGKEFDQRLEELGGKRISPRVDCDLDYDEPAAEWLEGVLKGLNEAGGGSAAPAPAAASQTGESSYSRTNPFRAEVLENLNLNGRGSNKETRHVELSLEGSGLTYEPGDSLGVYPENDPELVELLLKEMNWDPEEIVTLNKQGDVRPLKEALISHYEITVLTKPLLEQAAQLTGNDELRELLAPGNEENVKAYIEGRDLLDLVRDYGPFSVSAQEFVSILRKMPARLYSIASSLSANPDEVHLTIG------AVRYDAH-GRE---RKGVCS-ILCAERLQPGDTLPVYVQHNQNFKLPK-DPETPIIMVGPGTGVAPFRS-----FMQEREETGAEGKAWMFFGDQHFVTDFLYQTEWQNWLKDGVLT-KMDVAFSRDTEEKVYVQHRMLEHSAELFEWLQEGAAVYICGDEKHMAHDVHNTLLEIIEKEGNMSREEAEAYLADMQQQKRYQRDVY 3LO8 Chain:A ((85-311)) ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------VRLYSIASTRYG--DNFDGRTGSLCVRRAVYYDPETGKEDPSKNGVCSNFLCNSK--PGDKIQLTGPSGKIMLLPEEDPNATHIMIATGTGVAPFRGYLRRMFMEDVPNYRFGGLAWLFLGVAN-SDSLLYDEEFTSYLKQYPDNFRYDKALSREQ---MYVQDKIEEYSDEIFKLLDGGAHIYFCG-LKGMMPGIQDTLKKVAERRG----ESWDQKLAQLKKNKQWHVEVY

General information: TITO was launched using: RESULT: Template: 3LO8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 833 18663 22.40 92.39 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 22.40 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.443

(partial model without unconserved sides chains): PDB file : Tito_3LO8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3LO8-query.scw PDB file : Tito_Scwrl_3LO8.pdb: