Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKKTLKDIDVKGKVVFCRVDFNVPMKDGEVTDDTRIRAALPTIKHLADQGAKVLLASHLGRPKGEVVEELRLTPVAAR-LGELLGKEVKKADEAYGDAVKAQISEMKDGDVLVLENVRFYPGEEKNDP-------ELAKAFAELADVYVNDAFGAAHRAHASTAGIAEHLPAVAGFLMEKELDVLGKAVSNPDRPFTAIIGGAKVKDKIGVIESLL--DKVDNLIIGGGLAYTFVKALGYEVGKSLLEEDKIELAKSFMDRAKEKGVNFY----MPEDVLVADDFSNDANVKIVPISE---IPSDLEAIDIGTKTRETYADVIKNSKLVVWNGPMGVFEIDLFAQGTKAVAEALAEAKDTYSVIGGGDSAAAVEKFGLADKMSHISTGGGASLEFMEGKELPGVAALNDK 2CUN Chain:B ((4-399)) -----LEDFNFHNKTVFLRVDLNSPMKDGKIISDARFKAVLPTIRYLIESGAKVVIGTHQGKPYSE---DYTTTEEHARVLSELLDQHVEYIEDIFGRYAREKIKELKSGEVAILENLRFSAEEVKNKPIEECEKTFLVKKLSKVIDYVVNDAFATAHRSQPSLVGFARIKPMIMGFLMEKEIEALMRAYYSKDSPKIYVLGGAKVEDSLKVVENVLRRERADLVLTGGLVANVFTLAKGFDLGRKNVEFMKKKGLLDYVKHAEEILDEFYPYIRTPVDFAV--DYKGE-RVEIDLLSENRGLLHQYQIMDIGKRTAEKYREILMKARIIVANGPMGVFEREEFAIGTVEVFKAIADS-PAFSVLGGGHSIASIQKYGITG-ITHISTGGGAMLSFFAGEELPVLRALQ--

General information: TITO was launched using: RESULT: Template: 2CUN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2252 16526 7.34 43.60 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain B : 0.82 3D Compatibility (PKB) : 7.34 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.580

(partial model without unconserved sides chains): PDB file : Tito_2CUN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2CUN-query.scw PDB file : Tito_Scwrl_2CUN.pdb: