Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVAIASDHGGVHIRNEIKELMDELQIEYIDMGCDCGSGSVDYPDYAFPVAEKVVSGEVDRGILICGTGIGMSISANKVKGIRCALAHDTFSAKATREHNDTNILAMGERVIGPGLAREIAKIWLTTEFTGGRHQTRIGKISDYEEKNL 4LFN Chain:B ((1-128)) MIIAIGNDHIVTMQKIEISNMLKDMGYTVIDEGT-YDTHRTHYPIYGKKVAEDVADGRADLGIVMCGTGIGISTAADKNEGIRAAMCDDVTSAVYAREQLNANVLGIGGAVVGVHLIQDIVKAYLDATY--------------------

General information: TITO was launched using: RESULT: Template: 4LFN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 748 -96617 -129.17 -754.82 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain B : 0.78 3D Compatibility (PKB) : -129.17 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.529

(partial model without unconserved sides chains): PDB file : Tito_4LFN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LFN-query.scw PDB file : Tito_Scwrl_4LFN.pdb: