Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRNQMKIALLVGLAIVCIGLFLFYDLGNWDYTLPRRIKKVAAIVLTGGAIAFSTMIFQTITNNRILTPSILGLDSLYMLIQTGII--FLFGSANMVIMNKNINFIISVLLMILFSLVLYQIMFKGEGRNIFFLLLIGIVFGTLFSSLSSFMQMLIDPNEFQVVQDKMFASFNNINTDLLWLAFIIFLLTGVYVW--RFTKFFDVLSLGREHAVNLGIDYDKVVKQMLIVVAILVSVSTALVGPIMFLGLLVVNLAREFLKTYKHSYLIAGSVFISIIALVGGQFVVEKVFTFSTT-LSVIINFAGGIYFIYLLLKENKSW 4R9U Chain:A ((60-331)) ----------------------------WQIRLPRTL----AVLLVGAALAISGAVMQALFENPLAEPGLLGVSN---GAGVGLIAAVLLGQGQLPNWALGLSAIAGALIITLILL-----RFARRHLSTSRLLLAGVALGIISSALMTWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWL---MLALIPVLLWISSQSRPMNMLALGEISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILR-LSGLTDHRVLLPGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKA----

General information: TITO was launched using: RESULT: Template: 4R9U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1265 -243956 -192.85 -913.69 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -192.85 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.348

(partial model without unconserved sides chains): PDB file : Tito_4R9U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4R9U-query.scw PDB file : Tito_Scwrl_4R9U.pdb: