Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPYQINIANIKINGVTQNGNIDVGPTVHNSHTANSKYFGANFSLGDLSPTSSLLNTGNIDSDVSDQDQIGNPSAPISNQI 2HW2 Chain:A ((81-97)) --------------------------------------------------------IEDDPNVTDKKLPGNPT-------

General information: TITO was launched using: RESULT: Template: 2HW2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 24 4721 196.69 277.68 target 2D structure prediction score : 1.00 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : 196.69 2D Compatibility (Sec. Struct. Predict.) : 1.00 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.508

(partial model without unconserved sides chains): PDB file : Tito_2HW2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HW2-query.scw PDB file : Tito_Scwrl_2HW2.pdb: