Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTVLILNFPAEGHVNPTLGITKAFSDKGYDVHYISTEKYKKRLEAAGATVHLHRDLLR---TTPIHVGSPNGILDFVKIHIKTSLDILQIVKDLSKSIQFDFVYYD--KFGAGELVRDYLDIPGVSSSASFLFGE-------EHLKILPLHPESGAPLELDQEC---EDLLAKMKETYGVAPKNLVQFMNNKGELNVVYTSRYFQPESDRFGDEC-LFIGPSFPKRAEKTDFPIEQLKDEKVIYISMGTVLDHTEDFFNLCIDAFSGFNG-KVVIAAGEKADLTKLKQAPENFIIAPYVPQLEVLEQSDVFITHGGMNSVNEGIHFSVPLVVMPHDKDQPMVAQRLSELHAGYVISKDEVNAQILKQAVDEVLRNDQYTAGIKKINQSFKECMDMEEVMERIDELIRQKNK 2IYF Chain:A ((10-394)) ---IAMFSIAAHGHVNPSLEVIRELVARGHRVTYAIPPVFADKVAATGPRPVLYHSTLPGPDADPEAWGS--TLLDNVEPFLNDAIQALPQLADAYADDIPDLVLHDITSYPARVLARRW-GVPAVSLSPNLVAWKGYEEEVAEPMWREPRQTERGRAYYARFEAWLKENGITEHPDTFASHPPR-----------SLVLIPKALQPHADRVDEDVYTFVGACQ-----EGGW-QRPAGAEKVVLVSLGSAFT-QPAFYRECVRAFGNLPGWHLVLQIG---TPAELGELPDNVEVHDWVPQLAILRQADLFVTHAGAGGSQEGLATATPMIAVPQAVDQFGNADMLQGLGVARKLATEEATADLLRETALALVDDPEVARRLRRIQAE----MAQEGGTRRAADLI-----

General information: TITO was launched using: RESULT: Template: 2IYF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1861 -224325 -120.54 -624.86 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -120.54 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.456

(partial model without unconserved sides chains): PDB file : Tito_2IYF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2IYF-query.scw PDB file : Tito_Scwrl_2IYF.pdb: