Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPIGPGSLAVIAIVALIIFGPKKLPELGKAAGDTLREFKNATKGLTSDEEEKKKEDQ 2MI2 Chain:A ((4-41)) --IGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRS-----------------

General information: TITO was launched using: RESULT: Template: 2MI2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 29 -5836 -201.24 -153.58 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -201.24 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.404

(partial model without unconserved sides chains): PDB file : Tito_2MI2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2MI2-query.scw PDB file : Tito_Scwrl_2MI2.pdb: