Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLKKVILAAFILVGSTLGAFSFSSDAS-AKHVNGNITWYNGVGKKGSSGKKLGHWDCATKIGFDVPRNGTKIRAYAKAKPKKVITVYKNDVGRMPNAVLDVSPKAFKALGYPLSKGKVAGHYSY 3QO4 Chain:A ((80-163)) MIEKLPCAALTDRECTCPPGMFQSNATCAPHTVCPVGW--GVRKKGTETEDVRCKQCARGTFSDVPSSVMKCKAYTDCLSQNLVVI--------------------------------------

General information: TITO was launched using: RESULT: Template: 3QO4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 261 -10791 -41.34 -130.01 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -41.34 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.144

(partial model without unconserved sides chains): PDB file : Tito_3QO4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3QO4-query.scw PDB file : Tito_Scwrl_3QO4.pdb: