Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYVGRDMSELNMVSKKDWKNSELAYFHH----AFQQIMP---YLNEEGQSKYRELTQEIEARGGMKRNEADYSHGTRVSYD 2Z1S Chain:A ((78-119)) ---------------------QLGFLHYRFSVAWPRIMPAAGIINEEGLLFYEHLLDEIELAG------------------

General information: TITO was launched using: RESULT: Template: 2Z1S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 39 -3777 -96.83 -107.90 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -96.83 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.281

(partial model without unconserved sides chains): PDB file : Tito_2Z1S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Z1S-query.scw PDB file : Tito_Scwrl_2Z1S.pdb: