Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIKRYTCQNGVRIVLENNPTVRSVAIGVWIGTGSRHETPEINGISHFLEHMFFKGTSTKSAREIAESFDRIGGQVNAFTSKEYTCYYAKVLDEHANYALDVLADMFFHSTFDENELKKEKNVVYEEIKMYEDAPDDIVHDLLSKATYGNHSLGYPILGTEETLASFNGDSLRQYMHDYYTPDRVVISVAGNIS-DSFIKDVEKWFGSY---EAKGKATGLEKPEFHTEKLTRKKETEQA-HLCLGFKGLEVGHERIYDLIVLNNVLGGSMSSRL-FQDVREDKGLAYSVYSYHSSYED-SGMLTIYGGTGANQLQQLSETIQETLATLKRDGITSKELENSKEQMKGSLMLSLESTNSKMSRNGKNELLLGKHKTLDEIINELNAVNLERVNGLARQLFTEDYALALISPSGNMPS 3EOQ Chain:A ((7-405)) ------LRNGLRVIAEVVPGARSVALGYFVKTGARDETKEESGVSHFLEHMVFKGPEDMDALAVNRAFDRMGAQYNAFTSEEATVYYGAVLPEFAYDLLGLFAKL-LRPALREEDFQTEKLVILEEIARYQDRPGFMAYEWARARFFQGHPLGNSVLGTRESITALTREGMAAYHRRRYLPKNMVLAATGRVDFDRLLAEAERLTEAWPEGEAE-RAYPPLTPAFGVEE--RPYEKARALYLVALFPGVAYQEEARFPGQVLAHLLGEEGSGRLHFALV--DKGLA-EVASFGLEEADRAGTFHAYVQADPARKGEVLAVLQEELDRLGREGVGEEEVERAKTPLATGLVFAGETPMQRLFHLGMEYLYTGRYLSLEEVKARVQRVTSREVNALLERGFLEKGLYYLVLPHG----

General information: TITO was launched using: RESULT: Template: 3EOQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2080 -177964 -85.56 -453.99 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -85.56 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.495

(partial model without unconserved sides chains): PDB file : Tito_3EOQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EOQ-query.scw PDB file : Tito_Scwrl_3EOQ.pdb: