Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIAIYVRVSTEEQAIKGSSIDSQIEACIKKA---GTKDVLKYADEGFSGEL-LERPALNRLREDASKGL--ISQVICYDPDRLSRKLMNQLIIDDELRKRNIPLIFVNG-EYANSPEGQLFFAMRGAISEFEKAKIKERTSSGRLQKMKKGMIIKDSKLYGYKFVKEKRTLEILEEEAKIIRMIFNYFTDHKSPFFGRVNGIALHLTQMGVKTKKGAKVWHRQVVRQILMNSSYKGEHRQYKYDTEGSYVSKQAGNKSIIKIRPEEEQITVTIPAIVPAEQWDYAQELLGQSKRKHLSISPHNYLLSGLVRCGKCGNTMTGKKRKSHGKDYYVYTCRKNYSGAKDRGCGKEMSENKLNRHVWGEIFKFITNPQKYVSFKEAEQSNHLSDELELIEKEIEKTKKGRKRLLTLISLSDDDDLDIDEIKAQIIELQKKQNQLTEKCNEIQSKMKVLDDTSSSENALKRAIDYFQSIGADNLTLEDKKTIVNFIVKEVTIVDSDTIYIETY 3GUV Chain:A ((7-155)) KVYLYTRVSTSIQI-EGYSLEAQKSRMKAFAIYNDYEIVGEYEDAGKSGKSIEGRIQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIMQDYGVNLICVEDGIDSSKD--KLMISVLSAVAEIERENIRIQTMEGCIQKARE---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3GUV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 555 7751 13.96 55.36 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : 13.96 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.502

(partial model without unconserved sides chains): PDB file : Tito_3GUV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3GUV-query.scw PDB file : Tito_Scwrl_3GUV.pdb: