Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIRFAIIGTNWITDRFLESAADI--EDFQLTAVYSRSAERAGEFAAKHNAAHAFSDLQEMAASDCFDAVYIASPNALHKEQAVLFMNHGKHVLCEKPFASNTKETEEMISAAKANGVVLMEAMKTTFLPNFKELKKHLHKIGTVRRFTASYCQYSSRYDAFRSGTVLNAFQPELSNGSLMDIGVYCIYPAVVLFGA--PKDVKANGYALSSGVDGEGTVILSYDGFEAVLMHSKISTSYAP-----AEIQGEDGTI-VIDTIHRPERVEIRYRDGRLENIAIPDPK-------PAMFYEAEEFVTLIKENKLESEENTFERSLTTAKIMEEARKQMGIVYPADQA 3OHS Chain:X ((3-332)) -LRWGIVSVGLISSDFTAVLQTLPRSEHQVVAVAARDLSRAKEFAQKHDIPKAYGSYEELAKDPNVEVAYVGTQHPQHKAAVMLCLAAGKAVLCEKPMGVNAAEVREMVTEARSRGLFLMEAIWTRFFPASEALRSVLAQ-GTLGDLRVARAEFGKNLT-----HVPRAVDWAQAGGALLDLGIYCVQFISMVFGGQKPEKISVMGRRHETGVDDTVTVLLQYPGE----VHGSFTCSITAQLSNTASVSGTKGMAQLLNPCWCP--TELVVKGEHKEFLLPPVPKNCNFDNGAGMSYEAKHVRECLRKGLKESPVIPLVESELLADILEEVRRAIGVTFPQD--

General information: TITO was launched using: RESULT: Template: 3OHS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain X - contact count / total energy / energy per contact / energy per residue : 1827 -16398 -8.98 -52.39 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain X : 0.77 3D Compatibility (PKB) : -8.98 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.521

(partial model without unconserved sides chains): PDB file : Tito_3OHS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3OHS-query.scw PDB file : Tito_Scwrl_3OHS.pdb: