Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFMFEKPHGMRDTLP---GLYETKKKVRRSLTDLIDKWGYQFMETPTLE----FYDTVGVQSAIEEQQLFKLLDQ-DGKTLVLRPDMTGPIARVAASKLLKHGHPLRVGYAANVFRAQEREGGRPAEFEQVGVELIGDGTTSADAEVIALVVGALKNAGLASFKIAIGHAGIAD---ALFVEVLGNVER-ADVLRRFLYEKNYVGYREHVKSLPLSSID-KSRLLELLELRGGIEVCGRAEEIVDSAQGKSVVDELKALWDILEDYGCTENVRLDLN--MVSHMSYYTGILFEVYAENVGF-VIGSGGRYNKLLGHF-DSPAPATGFGLRIDRLIEAL---HMKDEPCEIDAVIFSKEQRAQAIAYANEERMK--GNKVVLQ---DLSGIENIDQMTKSFANVTYFIGARKEEQNG 4E51 Chain:A ((37-420)) --------GMNDILPQDAGLWEFFEATVKSL---LRAYGYQNIRTPIVEHTPLFTRGIGEVTDIVEKEMYSFVDALNGENLTLRPENTAAVVRAAIEHNMLYDGPKRLWYIGPMFRHERP---RYRQFHQVGVEALGFAGPDADAEIVMMCQRLWEDLGLTGIKLEINSLGLAEERAAHRVELIKYLEQHADKLDDDAQRRLYTN--------PLRVLDTKNPALQ--------EIVRNAPKLID-----FLGDVSRAHFEGLQRLLKANNVPFTINPRLVRGLDYYNLTVFEWVTDK---GTVAAGGRYDPLIEQLGGKPTAACGWAMGIERILELLKEEHLVPEQEGVDVYVVHQGDAAREQAFIVAERLRDTGLDVILHCSADGAGASFKSQMKRADASGAAF----------

General information: TITO was launched using: RESULT: Template: 4E51.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1645 24308 14.78 70.87 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : 14.78 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.398

(partial model without unconserved sides chains): PDB file : Tito_4E51.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4E51-query.scw PDB file : Tito_Scwrl_4E51.pdb: