Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEGVENKVIVLAGMIGAGKSSYT----------ELIANELGTKAFYESIKD------------NRILEMFYDDPKRWAFALQIYFLNTRFR----SIKAALTDQNN---VLDRSIYEDA-LFTQINFEEGNISEPEMDTYLDLLDNMMEELAYMPKKAPDLLIYLRGSLDTVLSRIALRGRPYEQIDDNPGL-LEYYKHLHSRYDSWFASYDKSETLVINIDEIDINKPNDADYVMQLIHEKLKR 2ZI3 Chain:A ((39-245)) ----RIKKISIEGNIAAGKSTFVNILKQLSEDWEVVPEPVARWSNVQSTQDEFEELTMSQKNGGNVLQMMYEKPERWSFTFQTYACLSRIRAQLASLNGKLKDAEKPVLFFERSVYSDRYIFASNLYESESMNETEWTIYQDWHDWMNNQFG--QSLELDGIIYLQATPETCLHRIYLRGRNEEQ-----GIPLEYLEKLHYKHESWLL----HRTLKTNFD-----------------------

General information: TITO was launched using: RESULT: Template: 2ZI3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -78150 for 1243 contacts (-62.9/contact) + 2D Compatibility (PS) -18968 + (NN) -7512 + (LL) 2252 1D Compatibility (HY) -13600 + (ID) 3100 Total energy: -119078.0 ( -95.80 by residue) QMean score : 0.379

(partial model without unconserved sides chains): PDB file : Tito_2ZI3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZI3-query.scw PDB file : Tito_Scwrl_2ZI3.pdb: