Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MELVIRDIRKRFQETEVLRGASYRFYSGKITGVLGRNGAGKTTLFNILYGDLAADNGTICLLKDNHEYPLTDK-------DIGIVYSENYLPEFLTG-YEFVKF---YMDLHPSDDLMT-IDDYLDFMEIGQTERHRIIKGYSDGMKSKLSLICLMISKPKVILLDEPLTAVDVVSSIAIKRLLLELSEDH--IIILSTHIMALAEDLCDIVAVLDKGKLQTLDIDRKHEQFEERLLQVLKGDEYDK 3GFO Chain:A ((21-222)) --------------THALKGINMNIKRGEVTAILGGNGVGKSTLFQNFNGILKPSSGRI--LFDNKPIDYSRKGIMKLRESIGIVFQDPDNQLFSASVYQDVSFGAVNMKL-PEDEIRKRVDNALKRTGI-EHLKDKPTHCLSFGQKKRVAIAGVLVMEPKVLILDEPTAGLDPMGVSEIMKLLVEMQKELGITIIIATHDIDIVPLYCDNVFVMKEGRVILQGNPKEVFAEKEVIRKVNLRLPRIG

General information: TITO was launched using: RESULT: Template: 3GFO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -143409 for 1366 contacts (-105.0/contact) + 2D Compatibility (PS) -20147 + (NN) -8046 + (LL) 1836 1D Compatibility (HY) -12800 + (ID) 3050 Total energy: -185616.0 ( -135.88 by residue) QMean score : 0.353

(partial model without unconserved sides chains): PDB file : Tito_3GFO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3GFO-query.scw PDB file : Tito_Scwrl_3GFO.pdb: