Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKNDQIVFEKTKNIAHDINQMQNQQEIIDYLFRQDSLTLNQLKHYYSEPSLPLQFLVKVAVLCMFISMTLASFLFIQAKEVFTNTILSDISPAVFSIFTVICIFMTYTKIIKKGNKNKGKASLNQRSEFYEKNKLINTILYKKYKMDQQNIQANKHTASDNEDSMNFSAVLNHVLTISKNDKELLGYLDTRDNAMLSQLKAYFSTRPFSLPHYMSLMFCGSIIVVYATSLFSGQINYIDIPHIFIFLLLIIFLKILIDLIKLLNISRKGQLHTVLHFAQRAEYLRMRGVIDFILTERYNKKIM 4N1L Chain:A ((8-38)) --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SFFPDFGLLLYLEELNKEELNTFKLFLKETM------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4N1L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 19 -2401 -126.37 -77.45 target 2D structure prediction score : 0.87 Monomeric hydrophicity matching model chain A : 0.47 3D Compatibility (PKB) : -126.37 2D Compatibility (Sec. Struct. Predict.) : 0.87 1D Compatibility (Hydrophobicity) : 0.47 QMean score : 0.553

(partial model without unconserved sides chains): PDB file : Tito_4N1L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4N1L-query.scw PDB file : Tito_Scwrl_4N1L.pdb: