Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLITVPLAGELKFYPLNEEFRVSFGAPVFFFFLSLLRHVPAVLPGFLTGAAVFIFRVFLELWGGGHNGLTPILYDQASGFFFYMTYACLFSILKANRFRERPIMLGFIGFMIEVVSDCVELTVQFLIFHTVVTPEKITDIAVIAISHTFIVMSFYSVLKLYETQSREKQTRQQHEHMLMIVSNLYEETVHLKKTLKTTEKVTNDSYQLYREMKGKDVQLSGRILRLAGEIHEVKKDNQRIFAGLSKLISNESLRDYMRASDLLQLVIRMNEKYAEALGKQIDFYCSIEGEHDEYHVFIVLSIINNLTANAVEAMDEEGMVSLRLRKPNESM---VEFQVEDNGPGISEKIGDIVFDPGFTSKYDEFGTPSTGIGLSYVKEIV-TELEGDITFDNQQRGVVFAIRLPVRHLIQKG 3A0Z Chain:A ((651-749)) ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------IKQVLINLVQNAIEATGENGKIKI----TSEDMYTKVRVSVWNSGPPIPEELKEKIFSPFFT----------TGLGLSICRKIIEDEHGGKIWTENRENGVVFIFEIP--------

General information: TITO was launched using: RESULT: Template: 3A0Z.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 378 -50724 -134.19 -563.59 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.53 3D Compatibility (PKB) : -134.19 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.53 QMean score : 0.578

(partial model without unconserved sides chains): PDB file : Tito_3A0Z.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3A0Z-query.scw PDB file : Tito_Scwrl_3A0Z.pdb: