Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAVVRATSADVDLMARLLRAEAEGEGKQGMLLVGNVGINRLRANCSDFKGLRTIRQMIYQPHAFEAVTHGYFYQRARDSERALAR--RSIN-GERRWPAKFSLWYFRPQGD-----CPAQWYNQPFVARFKSHCFYQPTAETCENVYNTF 2IS4 Chain:A ((116-195)) ---------------------------------------------------------------------------SEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ---NPVEQTWQK--VYQAYQEACD------

General information: TITO was launched using: RESULT: Template: 2IS4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 121 -13154 -108.71 -234.89 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -108.71 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.563

(partial model without unconserved sides chains): PDB file : Tito_2IS4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2IS4-query.scw PDB file : Tito_Scwrl_2IS4.pdb: