Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEHLPEQYRQLFPTLQTHTMLASCSQSALAEPVSRAIQDYYDSLL--YKGTNWKEAIEKTEFARNEFAKLIGAEPDEVAIVPSVSDALVSVASSL-----TAFGKKHVVYTDM-DFPAVPHVWQAHSD-----YTVSVIPSIDGVLPLEQYETHISDETVLTCVPHVHYRDGYVQDIKAIAEISQRKGSLLFVDAYQSAGHIPIDVKEWGVDMLAAGTRKYLLGIPGV-AFLYVRKELADALKPKASAWFG--RESGFDGAY--AKVARRFQTGTPAFISVYAAAAALSLLNHIGVSHIRDHVKTICADAVQYAAEKGLQLAAAQGGIQPGMVAIRDERASETAGLLKKKKVICAPRENVIRLAPHFYNTKEEMRHAIDEIAAKTIHK 1QZ9 Chain:A ((43-307)) --------------------------AALARAQAVIAEEWGNGLIRSWNSAGWRDLSERLG---NRLATLIGARDGEVVVTDTTSINLFKVLSAALRVQATRSPERRVIVTETSNFPTDLYIAEGLADMLQQGYTLRLVDSP------EELPQAIDQDTAVVMLTHVNYKTGYMHDMQALTALSHECGALAIWDLAHSAGAVPVDLHQAGADYAIGCTYKYLNGGPGSQAFVWVSPQLCDLVPQPLSGWFGHSRQFAMEPRYEPSNGIARYLCGTQPITSLAMVECGLDVFAQTDMASLR----------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1QZ9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1315 -171871 -130.70 -695.83 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -130.70 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.421

(partial model without unconserved sides chains): PDB file : Tito_1QZ9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1QZ9-query.scw PDB file : Tito_Scwrl_1QZ9.pdb: