Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHVITTQVLFIFCFLLLIHSIETLAYATRLSGARVGFIASALSLFNVMVIVSRMSNMVQQPFTGHLIDDAGKNALAIVGEQFRFLIFGSTVGTILGIILLPSFVALFSRAIIHLAGGGGSVFQVFRKGFSKQGFKNALSYLRLPSISYVKGFHMRLIPKRLFVINMLITSIYTIGVLSALYAGLLAPERSTTAVMASGLINGIATMLLAIFVDPKVSVLADDVAKGKRSYIYLKWTSVTMVTSRVAGTLLAQLMFIPGAYYIAWLTKWF 2VJ6 Chain:A ((321-349)) ---------------------------------------------------------------------------------YKFAISQSSTGTVMGAVIMEGFYVVFDRA---------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2VJ6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 20 -5081 -254.03 -175.19 target 2D structure prediction score : 0.10 Monomeric hydrophicity matching model chain A : 0.42 3D Compatibility (PKB) : -254.03 2D Compatibility (Sec. Struct. Predict.) : 0.10 1D Compatibility (Hydrophobicity) : 0.42 QMean score : 0.145

(partial model without unconserved sides chains): PDB file : Tito_2VJ6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2VJ6-query.scw PDB file : Tito_Scwrl_2VJ6.pdb: