Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKISFSESQKLFAYFSGLIAALSLFIYYVSAQQSEGALILCITFGVIAAGIWFGPIYALAVTLIVLFVLGTLMMFFQTGQTSLFPAEEGLRMLVVWGIALLLFSFISGRIHDITAELRRSMTRLQSEIKSYVAVDRVTGFDNKQRMKLELSEEIKRAERYGNSFVFLLLHMHYFKEFKSLYGEKETDRLFQYVGQQIRTSV-RETDKKFRPSDERIGIVLTHTPAEHMPAVLTKLKKQLDTYQLEN-----GKYVSLTFHVCYL-------PYRNDIQTADQFLEELENEMMMNEL 3I5B Chain:A ((6-160)) --------------------------------------------------------------------------------------------------------------------------------------DGLTGLSNRRHFDEYLEMEWRRSLREQSQLSLLMIDVDYFKSYNDTFGHVAGDEALRQVAGAIREGCSRSSDLAARYGGEEFAMVLPGTSPGGARLLAEKVRRTVESLQISHDQPRPGSHLTVSIGVSTLVPGGGGQTFRVLIEMADQALYQAKN-------

General information: TITO was launched using: RESULT: Template: 3I5B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 690 -89383 -129.54 -629.45 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : -129.54 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.597

(partial model without unconserved sides chains): PDB file : Tito_3I5B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3I5B-query.scw PDB file : Tito_Scwrl_3I5B.pdb: