Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSFWKNKNVFVTGCTGLLGSYLVKELIEQGANVTGLVRDHVPQSNLYQGEHIKKMN----IVRGSLEDLAVIERALGEYEIDTVFHLAAQAIVGVANRNPISTFEANILGTWNILEACRKHPLIKRVIVASSDKAYGDQ-ENLPYDENMPLQGKHPYDVSKSCADLISHTYFHTYGLPVCITRCGNLYGGGDLNFNR--IIPQTIQLVLNGEAPEIRSDGTFVRDYFYIEDAVQAYLLLAEKMEENNLAGEAFNFSNEIQLTVLELVEKILKKMNS-NLKPKVLNQGSNEIKHQYLSAEKARKLLNWTPAYTIDEGLEKTIEWYTEFFKK 4ZRN Chain:A ((5-307)) -------NILVTGGAGFIGSHVVDKLIENGYGV--IVVD-----NLSSGK-VENLNRNALFYEQSIEDEEMMERIFSLHRPEYVFHLAAQASVAISVREPARDAKTNIIGSLVLLEKSIKYGVKKFIFSSTGGAIYGENVKVFPTPETEIPHPISPYGIAKYSTEMYLEFFAREYGLKYTVLRYANVYGPRQDPYGEAGVVAIFTERMLRGEEVHIFGDGEYVRDYVYVDDVVRANLLAMEKGDN-----EVFNIGTGRGTTVNQLF-KLLKEITGYDKEPVYKPPRKGDVRKSILDYTKAKEKLGWEPKVSLEEGLKLTVEYF------

General information: TITO was launched using: RESULT: Template: 4ZRN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1703 -150844 -88.58 -511.33 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -88.58 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.525

(partial model without unconserved sides chains): PDB file : Tito_4ZRN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZRN-query.scw PDB file : Tito_Scwrl_4ZRN.pdb: