Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTASQQQIQLARRPQGIPVHEDFRFET----IPVPEPKQGEVLVKTLYVSVDPYMRGRM--QDTKSYVEPFALDKALSGGVIAEVVSDGN-HLKKGDIVIGNLSWQEFSAVS--ESALRKIDTSLAPASAYLGILGMTGLTAYFGLLDIGRPKEGETVVVSGAAGAVGSTVGQIAKIKGARVVGIAGSDEKIDYLKQELQFDEAINYKTADDIQKALQNACPDGVDVYFDNVGGPISDAVMNLLNEFARIPVCGAISSYNAESEADDMGPRVQSKLIKTKSLMQGFIVSDYSDRFSEGAKQLAEWLKAGKLHYEETITEGFENIPDAFLGLFKGENKGKQLIKVSDPS 2J3K Chain:A ((1-343)) MTATNKQVILKDYVSGFPTESDFDFTTTTVELRVPE-GTNSVLVKNLYLSCDPYMRIRMGK-------QAYTPGQPIQGYGVSRIIESGHPDYKKGDLLWGIVAWEEYSVITPMTHAHFKIQHTDVPLSYYTGLLGMPGMTAYAGFYEVCSPKEGETVYVSAASGAVGQLVGQLAKMMGCYVVGSAGSKEKVDLLKTKFGFDDAFNYKEESDLTAALKRCFPNGIDIYFENVGGKMLDAVLVNMNMHGRIAVCGMISQYNLENQE---GVHNLSNIIYKRNRIQGFVVSDFYDKYSKFLEFVLPHIREGKITYVEDVADGLEKAPEALVGLFHGKNVGKQVVVVA---

General information: TITO was launched using: RESULT: Template: 2J3K.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1907 -185963 -97.52 -572.19 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.82 3D Compatibility (PKB) : -97.52 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.487

(partial model without unconserved sides chains): PDB file : Tito_2J3K.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2J3K-query.scw PDB file : Tito_Scwrl_2J3K.pdb: