Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMKKLFHSTLIVLLFFSFFGVQPIHAKKQFKVPNS---VASISKENTYPNASQDQPMLQPSKLAKELLDHSEVKIENPHLIKMLNESNISGTPLAVGYRATIFLGKWALGYESNETVANWEYKKINTNRADNRGGKETAEMHYAQEQQYRVKGGLTAKVPNAEDVKSMMMQKAMKKTNLPLAFETVIGAGTKRDQIYKVAPKKIGYLHAYAPAVNEKGKVTYGEVYLVLKGNKRKLVVKNVTSQGIGAWIPVQDHVTFGFQLSSLPR 4TY6 Chain:A ((72-123)) ----------------SFFGVQEIIIHDQYKMAESGYDIALLKLETTVGYGDSQRPICLPSKGDRNVI-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4TY6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 54 -2217 -41.05 -45.23 target 2D structure prediction score : 0.27 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : -41.05 2D Compatibility (Sec. Struct. Predict.) : 0.27 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.571

(partial model without unconserved sides chains): PDB file : Tito_4TY6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4TY6-query.scw PDB file : Tito_Scwrl_4TY6.pdb: