Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKKSFSIVIAGGGSTFTPGIVLMLLDHLEEFPIRKLKLYDNDKERQDRIAGACDVFIREKAPDIEFAATTDPEEAFTDVDFVMAHIRVGKYAMRALDEQIPLKYGV---VGQETCGPGGIAYGMRSIGGVLEILDYMEKYSPDAWMLNYSNPAAIVAEATRRLRPNSKILNICDMPVGIEDRMAQI----LGLS--SRKEMKVRYYGLNHFGWWTSIQDQEGNDLMPKLKEHVSQYGYIPKTEAEAVEASWNDT-FAKARDV------------QAAD---PDTLPNTYL------QYYLFPDDMVKKSNPNHTRANEVMEGREAFIFSQCDMITREQSSENSEIKIDDHASYIVDLARAIAYNTGERMLLI-VENNGAIANFDPTAMVEVPCIVGSNGPEPITVGTIPQFQKGLMEQQVSVEKLTVEAWAEKSFQKLWQALILSKTVPNARVARLILEDLVEANKDFWPELDQSPTRIS 3FEF Chain:A ((63-416)) ------------------------------------------------------------------YEAVSTLKKALSAADIVIISILPGSLDDMEVDVHLPERCGIYQSVG-DTVGPGGIIRGLRAVPIFAEIARAIRDYAPESWVINYTNPMSVCTRVLYKVFPGIKAIGCCHEVFGTQKLLAEMVTERLGIEVPRREDIRVNVLGINHFTWITKASYRH-IDLLPIFREFSAHYG---ESGYELEGECWRDSVFCSAHRVAFDLFETYGAIPAAGDRHLAEFLPGPYLKQPEVWKFHLTPISFRKQD-----RAEKRQE-TERLIVQQRGVAEKASGEEGVNII-------------AALLGLGELVTNVNMPNQGQVLNLPIQAIVETNAFITRNRVQPILSGALPKGVEMLAARHISNQEAVADAGLTKDTGLAFQAFL-------------------------------------

General information: TITO was launched using: RESULT: Template: 3FEF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1491 -129502 -86.86 -402.18 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -86.86 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.413

(partial model without unconserved sides chains): PDB file : Tito_3FEF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FEF-query.scw PDB file : Tito_Scwrl_3FEF.pdb: