Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDYADY-DKALYYTHRS----QWDNLLILMVRTE--DDLLSKRIEHFLHAYHFEQDYAVLEKMLYSLLRYIDHATELTYEDQIALLT 4ES7 Chain:A ((133-185)) ---TNYDEYAIFLTKKFSRHHGPTITAKLYGRAPQLRETLLQDFRVVAQGVGIPED-------------------------------

General information: TITO was launched using: RESULT: Template: 4ES7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 108 -16436 -152.19 -357.30 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -152.19 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.336

(partial model without unconserved sides chains): PDB file : Tito_4ES7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ES7-query.scw PDB file : Tito_Scwrl_4ES7.pdb: