Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFKIKKKKLFIPIIILVLTAFLALIGYISIIFLGHYVIDEKKLILHASSKIVDQNGDEVASLYTENR-EPVSINEIPKQVREAFIAVEDKRFYEHHGIDAKSVGRAVYRDILAGGKVEGGSTITQQLAKNIFLTHDKTFLRKTKEVIIAINLERDYSKDKLLEMYLNQLYFGHGVYGIQAASHYYFNKEVKDLTVSEGAVLAAIPKAPSTYSPILHPDKNKERRDTILGMMNDQGYISAKEAVTAQGRTLGLHVKKQSETP------------WFDSYIDLVIKEAEDKYSISGEQL---LQGGYTIKVPLDSKLQKTAYQVMKEGSYYPGTDQNAEGSAVFINNKTGGVEAAIGGRDYTS-KGYNRVTAVRQPGSTFKPLAVYGPAMQEKKFKPYSLLKDELQSYGD-YTPKNYDSRYEGEVTMSDAITYSKNAPAVWTLNEI----GVETGKSYLKANGIDIP-DEGLALALGGLEKGVSPLQLAGAFHTFAANGTYTEPFFISSIIDEDGETIA-DHKEEGKRVFSKQTSWNMTRMLQQVVKK-----GTATSGTYHGDLAGKTGSTSYTGVS--------GATKDAWFAGYTPKITGAVWMGYDKTDQ---NHYL-KAGSSYPTRLFKDILTQ-AGETGHVFTKPKNVKELESPIELKPVKTLTADYTFKAAGLFTIELKWDAQEDDRAVYRIYVNKDGEETLLDSVEGKGSYEIPYANLFSGASYKIVPYNTQTKREGEGTDYVQPKLFSS 2OLV Chain:A ((9-611)) ----------------------------------------AKLQDPIPAKIYDKNGELVKTLDNGQRHEHVNLKDVPKSMKDAVLATEDNRFYEHGALDYKRLFGAIGK---------GASTLTQQVVKDAFLSQHKSIGRKAQEAYLSYRLEQEYSKDDIFQVYLNKIYYSDGVTGIKAAAKYYFNKDLKDLNLAEEAYLAGLPQVPNNYNIYDHPKAAEDRKNTVLYLMHYHKRITDKQWEDA--KKIDLKANLVNRTPEERQNIDTNQDSEYNSYVNFVKSELMNNKAFKDENLGNVLQSGIKIYTNMDKDVQKTLQNDVDNGSFYKNKDQQV--GATILDSKTGGLVAISGGRDFKDVVNRNQATDPHPTGSSLKPFLAYGPAIENMKWATNHAIQDESSYQVDGSTFRNYDTKSHGTVSIYDALRQSFNIPALKAWQSVKQNAGNDAPKKFAAKLGLNYEGDIGPSEVLGGSASEFSPTQLASAFAAIANGGTYNNAHSIQKVVTRDGETIEYDHTSH--KAMSDYTAYMLAEMLKGTFKPYGSAYGHGVSGVNMG---AKTGTGTYGAETYSQYNLPDNAAKDVWINGFTPQYTMSVWMGFSKVKQYGENSFVGHSQQEYPQFLYENVMSKISSRDGEDFKRPSSV--------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2OLV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3059 -119441 -39.05 -216.38 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -39.05 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.386

(partial model without unconserved sides chains): PDB file : Tito_2OLV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2OLV-query.scw PDB file : Tito_Scwrl_2OLV.pdb: