Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIQLSEDQIVKVTGDVSSPKGFQAKGVHCGLRYSKKDLGVIISETPAVSAAVYTQSHFQAAPIKVTQDSLKHGPTLKAVIVNSAIANACTGEQGLKDAYTMRESFASQLGIEPELVAVSSTGVIGEHLDMEKIHAGIELLKETPAGSGDFEEAILTTDTVIKQTCYELAIGGKTVTIGGAAKGSGMIHPNMATMLGFVTTDAAIEEKALQKALREITDVSFNQITVDGETSTNDMVLVMANGCAENECLTEDHPDWPVFKKALLLTCEDLAKEIARDGEGATKLIEAQVQGAKNNLDANVIAKKIVGSNLVKTAVYGTDANWGRIIGAIGHSA--AQVTAEEVEVYLGGQCLF--KNNEPQPFSESIAKEYLEGDEITIVIKMAEGDGNGRAWGCDLTYDYIKINASYRT 2YEP Chain:F ((1-212)) ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TLLTFFATDARLDPAEQDRLFRRVMDRTFNAVSIDTDTSTSDTAVLFANGLAG-------EVDAGEFEEALHTAALALVKDIASDGEGAAKLIEVQVTGARDDAQAKRVGKTVVNSPLVKTAVHGCDPNWGRVAMAIGKCSDDTDIDQERVTIRFGEVEVYPP-------------AEHLRGDEVVIGIDLAIADGAFTVYGCDLTEGYVRLNSEYT-

General information: TITO was launched using: RESULT: Template: 2YEP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 897 -114245 -127.36 -591.94 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain F : 0.66 3D Compatibility (PKB) : -127.36 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.390

(partial model without unconserved sides chains): PDB file : Tito_2YEP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2YEP-query.scw PDB file : Tito_Scwrl_2YEP.pdb: